Resources and articles

Cryo-ET sample preparation becomes more demanding when specimens are thicker, larger, or more complex. This webinar brings together four perspectives on how high-pressure freezing can be connected with downstream cryo workflows, including waffle preparation, correlative cryo targeting, on-grid lamella preparation, cryo-lift-out, serial lift-out, and autogrid-compatible approa...

Organoids and other complex in vitro models (CIVMs) are becoming increasingly important in early drug discovery and translational research, driven by the need for more predictive, human-relevant data in line with evolving regulatory expectations. However, scientists face many challenges when imaging and analyzing these 3D systems. This eBook explores how complementary imaging...

Waffle freezing provides an HPF-based route to cryo-ET sample preparation. This workflow guide follows the process from grid and carrier assembly to vitrification, cryo-FIB milling, lamella optimization, and cryo-ET imaging — with a focus on the control points that influence reproducibility and lamella yield.