Infinita Biotech

Key Highlights Glucoamylase (exo-1,4-glucosidase) is the rate-limiting enzyme converting dextrins and oligosaccharides to fermentable glucose in ethanol production. Optimal batch saccharification parameters: 55 to 60°C, pH 4.0 to 4.5, dosage 0.5 to 1.5 AGU/g starch. Thermostable glucoamylase variants extend activity to 60 to 65°C, enabling tig...

Key Highlights Rennet is defined by its chymosin content: animal rennet contains 85 to 95% chymosin plus pepsin, while FPC delivers close to 100% pure chymosin. Microbial rennet from Rhizomucor miehei is cost-effective but introduces bitterness in long-aged cheeses due to broad proteolytic specificity. Fermentation-produced chymosin (FPC) acco...

Key Highlights Rhizomucor miehei is the primary commercial source of microbial rennet, producing aspartyl proteases that cleave kappa-casein to coagulate milk. Clotting activity is measured in IMCU (International Milk Clotting Units), with commercial preparations typically supplied at 400 to 1,000 IMCU per mL. Optimal activity occurs between pH 5.5 and...

Key Highlights Laccase (EC 1.10.3.2) is a blue multi-copper oxidase that reduces O2 to H2O while oxidising phenolic and non-phenolic substrates across four major industrial sectors. Laccase-mediator systems (LMS) using ABTS or HBT extend substrate range to non-phenolic compounds including lignin, indigo, azo dyes, and pharmaceutical micropollu...

Key Highlights Enzymatic glucose syrup production uses a two-stage process: liquefaction with thermostable alpha-amylase at 85 to 110C, then saccharification with glucoamylase at 55 to 60C. Dextrose Equivalent (DE) is the defining quality parameter, ranging from DE 20 maltodextrins to DE 95+ high-glucose syrups, each suited to distinct applications. St...