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Thanks so much Beth!! This makes sense and is helpful intuition.


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I install cellpose3 and tried cyto2 and cyto3 models. Both works very good for myofiber segmentation and to my surprise, cyto2 works a little bit more precise. Thank you very much for your advice. You solved my problem.


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Another option, is using Herbie Glünder's macro which is similar to the one shown in this thread:

//imagej-macro "myoSegmentation" (Herbie G., 16. Aug. 2026) ttl=getTitle(); run("Duplicat...

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Welcome to image.sc @Loorent

One approach you can take is splitting the channels and segmenting only in a specific one. I tried CellposeSAM (model: cpdino, niter: 2000, flow_threshold: 0.8) and you can compare how they look (from left to right: Red, Green, Blue channels)
<...


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Welcome to image.sc @Yaaqub1

You'll encounter that with quite a bit of software in Mac unfortunately. What you need to do is go to System Settings, there go to Privacy and Security, and at the end it should appear that it wants to open/install the software. If it doesn't appear,...


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